Dab tsi yog Cov txiaj ntsig tseem ceeb thiab cov kev siv thaj chaw ntawm Neutral Protease?

 

Neutral proteasesyog metalloproteinases uas ua haujlwm nyob rau hauv nruab nrab pH tej yam kev mob (zoo pH 6.0–8.0) thiab feem ntau yog muab los ntawm Bacillus polymyxa.

 

Lawv qhov chaw ua haujlwm tseem ceeb muaj zinc ions thiab kev txuag cov amino acid ib ntus (histidine{0}}glutamic acid-histidine). Lawv tshwj xeeb hydrolyze fibronectin, laminin, thiab hom IV collagen nyob rau hauv lub extracellular matrix, tab sis muaj kev ua haujlwm tsawg hauv degrading fibrous collagen.

 

 

1. Kev ua siab mos thiab ua haujlwm zoo

• Nws ua haujlwm zoo ntawm physiological pH (7.0-8.0) thiab kub (37 degree), zam kev puas tsuaj ntawm tes los ntawm qhov muaj zog acid / alkali ib puag ncig.
• Nws precisely degrades cell-matrix junction proteins, khaws cia cell nto receptors thiab intercellular junction lug, ho txhim kho cell kev ua si thiab kev ua haujlwm zoo.

 

2. Kev ruaj ntseg siab

Neutral protease enzymetsis hnov ​​​​tsw rau cov khoom xyaw hauv cov ntshav, thiab nws qhov ruaj khov tuaj yeem txhim kho los ntawm kev ntxiv Ca²⁺; chelating cov neeg ua haujlwm xws li EDTA tuaj yeem tshem tawm cov haujlwm enzyme sai sai, pab tswj cov tshuaj tiv thaiv.

 

3. Kev nyab xeeb zoo

Ua los ntawm cov kab mob fermentation, nws tsis muaj kev pheej hmoo ntawm mycoplasma lossis tsiaj kab mob kis, ua rau nws tsim nyog rau kev kho mob - qib cell npaj.

 

 

1. Kev Tshawb Fawb Txog Lub Neej thiab Kev Tshawb Fawb Txog Kev Kho Mob

• Primary Cell Isolation: Kev rho tawm ntawm neural stem cells, rho tawm ntawm cov hlwb epithelial
• Organoids thiab Cell Passaging
• Tissue Engineering: Kev rho tawm cov txheej txheem epidermal thiab dermal, los yog tso tawm ntawm epithelial lamellae los ntawm kab lis kev cai xov xwm rau kev tshawb fawb ntawm daim tawv nqaij.

 

2. Kev lag luam zaub mov

• Kev Lag Luam Ci: Hydrolyzes mov paj proteins, txo gluten lub zog, txhim kho biscuit plasticity thiab kev ntxhib los mos, thiab txhawb nqa Maillard cov tshuaj tiv thaiv los txhim kho tsw.
• Npias Clarification: Degrades cloudy proteins; Thaum ua ke nrog lwm cov enzymes, nws tuaj yeem tshem tawm iab.
• Protein Hydrolyzate Production: Npaj cov tsiaj txhu hydrolyzed thiab cog cov proteins siv rau hauv flavorings thiab khoom noj khoom haus fortifiers.

 

3. Pub Additives

Neutral protease hmoovtuaj yeem txhim kho kev siv tus nqi ntawm cov dej pub, txhawb cov metabolism hauv cov zaub mov thiab qhov hnyav nce hauv cov ntses.

 

 

Fruiterco yog lub tuam txhab xa tawm uas feem ntau tsim cov khoom cog, cov khoom noj khoom haus raw khoom, thiab cov khoom siv tshuaj pleev ib ce. Lub tuam txhab muaj 20+ kev pabcuam tom qab- pab pawg muag khoom.

 

Peb cov khoom raug muag rau North America, Europe, Australia, South America thiab Asia. Peb cov khoom tau dhau qhov kev tshuaj xyuas zoo thiab tseem txhawb cov neeg siv khoom rov- tshuaj xyuas. Yog koj xav tauyuav nruab nrab protease, thov hu rau peb:Email: info@fruiterco.com.

 

 

1. Albillos SM, Busto MD, Perez-Mateos M., Ortega N. (2007). Kev tshuaj xyuas los ntawm capillary electrophoresis ntawm kev ua haujlwm proteolytic ntawm aBacillus subtilisneutral protease ntawm bovine caseins. Int. Dairy J. 17, 1195–1200.

2. Belmessikh A., Boukhalfa H., Mechakra-Maza A., Gheribi-Aoulmi Z., Amrane A. (2013). Statistical optimization ntawm kab lis kev cai nruab nrab rau nruab nrab protease ntau lawm los ntawmAspergillus oryzae yog qhov txawv txav. Kev tshawb fawb sib piv ntawm cov khoom thiab submerged fermentation ntawm lws suav pomace. J. Taiwan. Inst. Chem. E 44, 377–385.

3. Campbell JM, Peberdy JF (1983). Cov khoom ntawm Aspergillus nidulans nruab nrab protease. FEMS Microbiol. Lett. 16, 49–53.

4. Eijsink VGH, van der Zee R., van den Burg B., Vriend G., Venema G. (1991). Txhim kho cov thermostability ntawm nruab nrab protease ntawmBacillus stearothermophiluslos ntawm kev hloov ib qho asparagine faus los ntawm leucine. FEBS Lett. 282, 13–16.

5. Ma X., Liu Y., Li Q., ​​Liu L., Yi L., Ma L., et al. (2016). Kev nthuav qhia, kev ua kom huv thiab kev txheeb xyuas ntawm ib qho thermolysin-zoo li protease, nruab nrab protease I, los ntawmAspergillus oryzae yog qhov txawv txavnrog rauPichia pastorisqhia system. Protein Expr. Purif. 128, 52–59.

6. https://draxe.com/nutrition/protease/#:~:text=Protease%20enzymes%20increase%20the%20potency, raws li%20to%20in%20vitro%20research.

7. Mouse islet ntawm langerhans cais siv kev sib xyaw ua ke ntawm purified collagenase thiab nruab nrab protease

8. Purification thiab characterization ntawm nruab nrab protease los ntawm Aspergillus oryzae Y1 cais los ntawm ib txwm fermented broad taum